RE’SUME’

Name:                           Qingxuan Chen
Profession:                    Professor
Address:                        Institute of Genetics and  Developmental Biology, Chinese Academy
of Sciences,P.O. Box 2707,Beijing 100080,China 
Phone Nnmber:             86-10-62553160;   86-10-82614427; Fax: 86-10-62551951
Email Address:             Click here to send me email                                

Education:
1965-1970:  Graduated from Peking University, Department of Biology; B.Sc.
1980-1983:  Graduated from Peking University, Graduate School, M. Sc.
1986-1989:  Visiting Scholar (equivalent to postdoctoral fellow), Washington University,
School of Medicine, St. Louis, USA

Research Experience:
1976-1983:  studies on purification of Cytochrome C and T4-RNA ligase with immunoaffinity          
chromatography; Peking University,Beijing.
1983-1986:  Purification and characterization of lectin from chicken embryo; and studies on the
seasonal changes of the steroid hormone level of Alligator Sinensis; Chinese Academy
of Sciences,Beijing.
1986-1989:  Purification and characterization of 3b,20a-hydroxysteroid dehydrogenase from  fetal
sheep blood, Washington University School of Medicine, St.Louis.
1989-1992:  Cloning of porcine growth hormone gene and atuding on transgenic pigs. Chinese Ac-
ademy of Sciences, Beijing.
1992-1993:   Cloning of the cDNA of sheep oestrus-associated oviductal glycoprotein (EGP). Division of
Animal Production, CSIRO, Sydeny.
1993- 1998:. Production  and identification of  transgenic  pigs. Chinese Academy of Sciences. Beijing.
1998-1999:  Gene expression and intermolecular forces in estrogen/receptor binding. Washington 
University School of Medicine,St.Louis.
1999-            Studies on dispelling  differentiation, reprogramming and developmental totipotency of
transplanted nucleus in the cytoplasm of oocyte. Chinese Academy of Sciences, Beijing.

Publications:

1,  Qingxuan Chen, Purification of T4-RNA ligase with immunoaffinity chromatography; Acta Zoologica 
Sinica,1986,32(3): 288-290.
2,  Yingxian Shi, Qingxuan Chen and Shipeng Li, The seasonal change of the steroid hormone of alligator
sinensis in raising environment, Acta Herpetologica Sinica,1986,5(2): 86-89.
3,   Ying-Hsien Shi and Qin-xue Chen (Qingxuan Chen), The specificity of chicken lactose lectin, Progress
in Developmental Biology, 1986,Part B, 47-50; Alan R.Liss,Inc.
4,   Qingxuan Chen ,Colin D.Nancarrow and Frederick Sweet, Isolation of 3b,20a-Hydroxysteroid Oxido-
reductase from sheep fetal blood, Steroids, 1987,49/6,477-496.
5,   Qingxuan Chen,Leonard O.Rosik, Colin D.Nancarrow and Frederick Sweet, Fetal lamb 3b,20a-Hydro-
xysteroid oxidoreductase: Dual activity at the same active site examined by affinity labeling with 16a-
(Bromo[2’-14C]acetoxy)progesterone, Biochemistry, 1989,28: 8856-8863.
6,   Qingxuan Chen and Yingxian Shi, The study of specificity of chicken embryo liver lactose lectin: An
application of SEM-ARC technique, Progress in Biochemistry and Biophysics, 1990, 17(6): 457-459.
7,   Qingxuan Chen, Colin D.Nancarrow  and Frederick Sweet,  Kinetic properties of 3b,20a-Hydroxys-
teroid dehydrogenase, Chinese Biochemical Journal, 1991,7(2):177-182.
8,   Qingxuan Chen,and Frederick Sweet, Identification of histidine residue in active site of fetal lamb 3b,
20a-Hydroxysteroid oxidoreductase, Progress in Biochemistry and Biophysics, 1991,18(2):113-116.
9,   Qingxuan Chen,Lei Liu,The application of PCR technique to study of transgenic animals, Progress in
Biotechnology, 1992, 19(3): 36-38.
10,  Qingxuan Chen et al. The transgenic mice of porcine growth hormone gene, Progress in Biochemistry
and Biophysics, 1992,19(3): 203-204.
11,  Qingxuan Chen and Zhihong Zhang, Isolation,purification and characterization of radish pigments, 
Chinese Biochemical Journal, 1993,9(4): 424-428.
12,  Qingxuan Chen et al.  High performance liquid chromatographic method for determination of enzyme
activity, Chinese Journal of Chromatography, 1992, 10(2): 101-103.
13,  Xiaoming Gong, Qingxuan Chen et al. Construction and screening of rabbit haemorrhangic disease
virus cDNA library.  Animal Biotechnology Bulletin, An official publication of the Asian Network for
biotechnology in animal production and health, 1992, (2): 8-11.
14,  Qingxuan Chen et al. In vitro culture of early rabbit embryos injected with foreign gene and its  
retention,  Developmental & Reproductive  Biology, 1993,2(2):62-68.
15,  Qingxuan Chen et al. Expression of porcine growth hormone gene in CHO cell, Developmental &
Reproductive Biology, 1994,3(2):34-39.
16,  Huinan Deng, Qingxuan Chen at al. Studies on exogenous growth hormone gene location of transgenic
pigs:  The technique of nonisotopic in situ hybridization on chromosome, Chinese Journal of    
Biotechnology, 1996,12(1):91-93.
17,  Guisheng Liu,Qingxuan Chen, et al.  Identification of integration of foreign gene in transgenic pigs,
Progress in Biochemical and Biophysics, 1996,23(3): 279-280.
18,  Qingxuan Chen,et al. Detection and analysis of an oestrus- associated oviductal glycoprotein DNA
fragments from primates by PCR. Developmental & Reproductive Biology, 1995,4(1): 1-5.
19,  Pinghua Wei and Qingxuan Chen , Transgenic Animal, 1996, Guangtong Scientific and Technology
Publishing House.
20,  Qingxuan Chen,et al. The construction of porcine growth hormone gene expressive plasmid and the
studies of transgenic animals, Chinese Biochemical Journal, 1997,13(5): 540-545.
21,  Qingxuan Chen,et al. Localization of an exogenous GH gene on chromosomes of transgenic pig by in
situ hybridization, Developmental & Reproductive Biology, 1997,6(1): 51-56.
22,  Zhihong Zhang, et al. The study on integration and expression of porcine growth hormone gene in
transgenic goldfish, Chinese Journal of Biotechnology;1998,14(2): 227-229.
23,  Qingxuan Chen,Stuart Adler and Frederick Sweet, Gene expression and intermolecular forces in 
estrogen/receptor binding, Proceedings of 10th International Congress on Genes,Gene Families and
Isozymes, October,1999,Beijing,China.
24,  Zou Shengwei, Chen Qingxuan, Inquiry to the research methods of genes’ differential display.Chinese
Bulletin of Life Sciences; 2000,12(2):80-81.
25,  Zou Shengwei,Chen Qingxuan, Molecular cloning of goat fetal liver cDNA encoding 3β-Hydroxys-
teroid dehydrogenase/△4-△5 isomerase;Developmental & Reproductive Biology,2001,10(1):
31-36.
26,Xu Qingyong, Lui Guisheng, Chen Qingxuan;Cloning of the goat(Capra hircus) Estrus- 
associated oviductual glycoprotein(gEGP) Gene;Chinese Journal of Biochemistry and  
Molecular Biology;2002,18(4): (in press).

About our Laboratory:

The laboratory called “Gene Engineering Laboratory” was founded in 1980 which research field is molecular developmental biology of mammal. In this laboratory ,the cDNA libraries were constructed for pig,chicken and fish and their growth hormone cDNA fragments were cloned respectively. One of the important projects(was supported by ‘863’ program) in this laboratory was to produce transgenic animals with growth hormone gene from different animals. In 1990,the growth hormone genimic gene was cloned from pig liver and then the studies were performed on integration, location,expression and regulation of foreign gene in transgenic  animals. Another project(supported by National Natural Science Foundation of China) is the isolation, purification  and characterization of 3β,20α-Hydroxysteroid Dehydrogenase(3β,20α-HSD) of fetal sheep and its gene cloning.  3β,20α-HSD exists just in fetal blood. When the baby  was  born, the  3β,20α-HSD  disappeared  soon in its blood. It is clear that the 3β,20α-HSD has certain  relationship with the development of the early embryo.  So, to study the molecular  mechanism of 3β.20α-HSD in  the embryo  is the main project in this Lab. Cloning of the goat estrus-associated oviductual glycoprotein(gEGP) gene is one of interesting project in this Lab and a part of gEGP gene has been cloned. A new project was started last year,that is “Studies on dispelling differentiation, reprogramming and developmental totipotency of transplanted nucleus in the cytoplasm of oocyte”,which was supported by the Major State Basic Research Development Program(called ‘973’).More recetly,some interesting  data were obtained with DDRT-PCR
Method and some differential fragments were identified which just appeared in the early stage of developmental embryo. The further studies of the differential fragments are carrying on. We are more interested in study of molecular mechanism of aging and anti-aging. In preparing  the studies, “SAM” mice  were imported from Japan last year. We have got some support for this field studies from “Ling Tai Chinese Traditional Medicine Company” and the studies would be started recently.